Reporter

Part:BBa_K1401611:Design

Designed by: Yash Agarwal   Group: iGEM14_BostonU   (2014-10-11)


pTet - BCD2 - RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 729
    Illegal AgeI site found at 841
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All basic BioBricks used were transformed into MoClo variants by adding the following fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. These were then put together using the digestion-ligation MoClo reaction. RFP Reporter (E1010m) has a fixed illegal cut site.


Source

E. Coli

References